October 2009: Dr. Martin Latterich joins PRISM as the first new faculty member. Dr. Latterich brings over 17 years of academic and technological experiences to PRISM. He and his laboratory focus on applying systems biology, genomics and proteomics to study cancer, degenerative disease and aging.
Recent Grant AwardsImmunotargeting Lung Cancer
Tobacco Related Disease Research Program
Dr. Jan E Schnitzer is the CO-PI of this grant which aims to use antibodies to specifically target lung tumors. Radioactive antibodies could be used to destroy tumors while sparing surround healthy tissue. Antibodies linked with imaging agents could be used to detect tumors and metastases and follow tumor progression. Ultimately, this work aims to drastically decrease the impact of lung cancer, both in terms of health care costs and in terms of human life.
Phase I – Small Hollow Silica Nanodelivery Vehicles for Cancer Treatment
National Cancer Institute, SBIR subcontract through nanoComposix
Dr. Philippe Valadon is a subcontract on this grant and will work with nanoComposix to create novel silica-based nanoparticles that will specifically target the breast cancer tumor antigen, Her2. Dr. Valadon will express and purify antibody fragments against the Her2 antigen that can be directly conjugated to the silica-based nanoparticles. These functionalized nanoparticles will be used to target chemotherapeutics to treat breast tumors in rodent models. Ultimately, this research will lead to safer and more effective nanoparticle-based therapies of human cancers.
Testa JE, Chrastina A, Li Y, Oh P, Schnitzer JE (2009) Ubiquitous yet Distinct Expression of Podocalyxin on Vascular Surfaces in Normal and Tumor Tissues in the Rat. J Vasc Res 46:311-324.
Li Y, Yu J, Wang Y, Griffin NM, Long F, Shore S, Oh P, Schnitzer JE (2009) Enhancing identifications of lipid-embedded proteins by mass spectrometry for improved mapping of endothelial plasma membranes in vivo. Mol Cell Proteomics 8:1219-1235.
Testa JE, Chrastina A, Oh P, Li Y, Witkiewicz H, Czarny M, Buss T, Schnitzer JE (2009) Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo. Am J Physiol Lung Cell Mol Physiol.
Halawani D, LeBlanc AC, Rouiller I, Michnick SW, Servant MJ, Latterich M (2009) Hereditary inclusion body myopathy-linked p97/VCP mutations in the NH2 domain and the D1 ring modulate p97/VCP ATPase activity and D2 ring conformation. Mol Cell Biol. 29: 4484-94.
Massey KA, Schnitzer JE (2009) Targeting and imaging signature caveolar molecules in lungs. Proc Am Thorac Soc 6:419-430.
Congratulations Ming!
Yi M, Schnitzer JE (2009) Impaired tumor growth, metastasis, angiogenesis and wound healing in annexin A1-null mice. Proc Natl Acad Sci U S A 106:17886-17891.
Congratulations to Philippe and his fellow researchers!
Valadon P, Darsow B, Buss TN, Czarny M, Griffin NM, Nguyen HN, Oh P, Chrastina A, Borgstrom P, Schnitzer JE. Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo. J Biol Chem, 285:713-722.
Congratulations to Noelle and her fellow researchers!
Griffin NM, Yu J, Long F, Oh P, Shore S, Li Y, Koziol JA, Schnitzer JE,
(2009) Label-free, normalized quantification of complex mass spectrometry data for proteomics analysis.
Nat Biotechnol, 28(1):83-9.
Congratulations to Adrian and his fellow researchers!
3. Chrastina A, Valadon P, Massey KA & Schnitzer JE. Lung vascular targeting using antibody to aminopeptidase P: CT-SPECT imaging, biodistribution and pharmacokinetic analysis. In press.
January 9, 2009
Angiogenesis and Lymphangiogenesis in Cancer
“Proteomic Imaging of Tumor Microenvironment and Angiogenesis In Vivo for Enhancing Targeted Therapies,” Keystone Symposia (Big Sky, Montana).
Speaker: Jan Schnitzer
January 21, 2009
24th Transatlantic Airway Conference
Imaging Pulmonary Pathology and Target Molecular Signatures, “Targeting and Imaging Signature Caveolar Molecules in the Lungs,” (Lucerne, Switzerland).
Speaker: Jan Schnitzer
February 13, 2009
Colorado State University
“In Vivo Proteomic Mapping of Endothelium and Caveolae for Drug Delivery Targeting Lung Diseases,” (Ft. Collins, CO).
Speaker: Jan Schnitzer
February 22-25, 2009
US HUPO 5th Annual Conference, San Diego, CA.
“Comprehensive proteomic analysis of lung endothelial caveolae identify novel functions for these membrane micro-domains,”
Poster Authors: Noelle M Griffin, P Oh, S Shore, H Witkiewicz, Y Li, J Yu, and JE Schnitzer
“Label-free, normalized quantification of complex mass spectrometry data for proteomics analysis,” Poster Authors: Noelle M Griffin, JYu, F Long, P Oh, S Shore, Y Li, JA Koziol, and JE Schnitzer
“Isolation of caveolae using detergent and detergent free approaches identifies specific proteins unique to each method yet localized to caveolae,”
Poster Authors: Noelle M. Griffin, P Oh, S Shore, H Witkiewicz, JE Schnitzer
“Functional proteomics analysis of lung endothelial plasma membranes identifies GPCRs which cause ligand induced budding of caveolae,”
Poster Authors: Noelle M. Griffin, P Oh, S Shore, H Witkiewicz, JE Schnitzer
“Enhancing identifications of lipid-embedded proteins by mass spectrometry for improved maping of endothelial plasma membranes in vivo,”
Poster Authors: Yan Li, J Yu, Y Peng Wang, F Long, S Shore, P Oh, and JE Schnitzer.
“Connecting proteomics and biological functions: Evaluation and systems analysis of the lung endothelial cell plasma membranome,”
Poster Authors: Yan Li, J Yu, NM Griffin, K Massey, and JE Schnitzer
March 3, 2009
Nobel Society Invitation Forum New Frontiers in Cancer Research and Therapy
“How to Target Tumors In Vivo: A Proteomic Imaging Strategy,” (Stockholm, Sweden).
Speaker: Jan Schnitzer
March 18-22, 2009
Systems Biology: Networks, Cold Spring Harbor, New York
“Comprehensive proteomic and systems analysis of lung endothelial caveolae identify novel functions for these membrane micro-domains,”
Poster Authors: Noelle M. Griffin, P Oh, S Shore, Y Li, J Yu, H Witkiewicz, JE Schnitzer
“Enhancing identifications of lipid-embedded proteins by mass spectrometry for improved maping of endothelial plasma membranes in vivo,”
Poster Authors: Yan Li, J Yu, Y Peng Wang, F Long, S Shore, P Oh, and JE Schnitzer.
Systems Biology: Networks, “Connecting proteomics and biological functions: Evaluation and systems analysis of the lung endothelial cell plasma membranome,”
Poster Authors: Yan Li, J Yu, NM Griffin, K Massey, and JE Schnitzer
April 22, 2009
FASEB: Experimental Biology
“Host microenvironment dictates tumor growth, angiogenesis and vascular permeability,” (New Orleans, LA).
Speaker: Phil Oh
June 12, 2009
35th Annual Meeting of Korean Cancer Association
“Proteomic Mapping of Endothelial Caveolar Signatures for Targeted Tumor Therapy in vivo,” plenary lecture (Seoul, Korea).
Speaker: Jan Schnitzer
August 12, 2009
2nd Salk Institute Mechanisms & Models of Cancer Meeting
“Proteomic Imaging of Tumor Microenvironment and Angiogenesis In Vivo For Enhancing Targeted Therapies” (San Diego, CA).
Speaker: Jan Schnitzer
September 27, 2009
Human Proteomic Organization (HUPO) 8th Annual World Congress
“Proteomic imaging of endothelium and caveolae for targeted penetration into single organs and solid tumors,” plenary lecture (Toronto, Canada).
Speaker: Jan Schnitzer
October 8, 2009
National Cancer Institute's 10th Principal Investigators Meeting for the IMAT
” Targeting vascular endothelial caveolae to pump antibodies into tumors for enhanced specific imaging and therapy,” (Bethesda, MD).
Speaker: Jan Schnitzer
October 22, 2009
National Cancer Institute Alliance for Nanotechnology in Cancer 4th Annual Investigators Meeting
“Mapping endothelial caveolae for tissue-specific nanodelivery in vivo,” (Manhattan Beach, CA).
Speaker: Jan Schnitzer
December 6-10, 2009
IBC’s Antibody Engineering and Antibody Therapeutics meeting
“Immunotargeted Delivery Across the Vascular Endothelium In Vivo,” (San Diego, CA).
Speaker: Jan Schnitzer
December 17-18, 2009
Image-Guided Drug Delivery Working Group to create white paper (Bethesda, MD)
Speaker: Jan Schnitzer
February 4-6, 2010
The 12th International Symposium on Anti-Angiogenic Agents
“Tumor Stroma and Targeted Delivery via Endothelial Caveolae,” (La Jolla, CA).
Speaker: Jan Schnitzer